Nick Kyrgios will open his Wimbledon campaign against Uzbekistan's Denis Istomin, while Bernard Tomic will play Pole Hubert Hurkacz after earning an unexpected late inclusion in the men's main draw.
Tomic suffered a final-round qualifying defeat but received a lucky loser entry just in time for Friday night's draw to give Australia 10 players in the men's singles at The All England Club.
Tomic's last-minute reprieve caught most off guard following the withdrawal of Spanish 14th seed Roberto Bautista-Agut.
Bautista-Agut's scratching also elevated Kyrgios from 16th to 15th in the seedings, with Australia's top title hope projected to run into German fourth seed Alexander Zverev in the fourth round.
Seeded 17th in the women's singles, Australian No.1 Ashleigh Barty will open against Swiss Stefanie Voegele and could face Russian 14th seed Daria Kasatkina in round three.
Tomic's good fortune aside and Kyrgios's relatively favourable run to the second week, it was a tough draw for most of Australia's 14-strong contingent.
James Duckworth was pitted against Zverev in round one, Matt Ebden drew Belgian 10th seed David Goffin, while Jordan Thompson struck big-serving American Sam Querrey, who has taken out defending champions Novak Djokovic and Andy Murray respectively over the past two years.
Surprise qualifier Alex Bolt will take on British 23rd seed Kyle Edmund, with exciting teenager Alex de Minaur to face French Open semi-finalist Marco Cecchinato, Italy's 23rd seed, in his main-draw debut on London's hallowed grass.
Daria Gavrilova, the 26th seed, plays Kazakh Zarina Diyas and could confront Fed Cup teammate Samantha Stosur in round two.
Stosur squares off with China's Peng Shuai first up, while Ajla Tomljanovic has a challenging opener against 10th seed Madison Keys in the American's first outing since hiring esteemed Australian coach David Taylor, Tomljanovic's one-time mentor.
HOW THE AUSSIES FARED IN THE WIMBLEDON DRAW (PREFIX DENOTES SEEDING)
Men's singles, first round
15-Nick Kyrgios v Denis Istomin (UZB)
James Duckworth v 4-Alexander Zverev (GER)
Matt Ebden v 10-David Goffin (BEL)
Jordan Thompson v 11-Sam Querrey
Alex Bolt v 23-Kyle Edmund (GBR)
Alex De Minaur v 29-Marco Cecchinato (ITA)
John Millman v Stefano Travaglia (ITA)
Bernard Tomic v Hubert Hurkacz (POL)
Jason Kubler v Guido Pella (ARG)
John-Patrick P Smith v Andreas Seppi (ITA)
17-Ashleigh Barty v Stefanie Voegele (SUI)
26-Daria Gavrilova v Zarina Diyas (KAZ)
Samantha Stosur v Peng Shaui (CHN)
Ajla Tomljanovic v 10-Madison Keys (USA)